FAB Lab - Software

Microtime Image Analysis (MIA)


MIA is a user friendly GUI for fluctuation analysis of images obtained with a microscope with PIE excitation and TCSPC detection, but any image obtained with an LSM can be analyzed also. The program depends on PAM (PIE analysis with matlab) when raw photon files are used, is a stand-alone program when TIF images are used, and is a standalone program when raw photon files are used, provided time gates, routing bits and module number are read-in properly. The main features of the program are:

  • Raster Image Correlation Spectroscopy
  • Cross correlation RICS
  • Global RICS analysis (multiple RICS images, multiple components, blinking)
  • Kinetic analysis
  • Z-scan imaging
  • Number & Brightness(N&B)
  • Cross N&B
  • Temporal and spatiotemporal ICS and ICCS
  • Lifetime filtering (removing dark counts, afterpulsing) from images or filtering for certain species on the basis of the lifetime (RLICS)
  • Serial analysis (hands-free analysis of multiple datasets)

Relevant literature

  Hendrix, Schrimpf, Hoeller and Lamb, 2013, Biophysical Journal 105
  Hendrix and Lamb, 2012, Methods in Enzymology 518
  Hendrix and Lamb, 2014, Methods in Molecular Biology 1075
  Hendrix, Baumgärtel, Schrimpf, Ivanchenko, Digman, Gratton, Kräusslich, Müller and Lamb (submitted)

Instructions for installing

You need a 64-bit windows pc to run the program.
The program supports multi-core CPUs with hyperthreading.
If for some reason you have no access to a 64-bit windows pc, please let us know.
  step 1: download and install the Matlab Runtime (MCRInstaller.exe)
  step 2: download and put the MIA_Vxxxx_pkg.exe file on a fixed location
  step 3: unpack by double clicking it
  step 4: Run the program by double clicking MIA_Vxxxx.exe
  step 5: Allow access to any process that asks your permission
  step 6(optional): you might want to put a shortcut to MIA_Vxxxx.exe on e.g. the desktop, for easy access.
Repeat steps 1-6 on any pc on which you want to install the program.

Click here to download the software

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