FAB Lab - Software

Actin Filament Dynamics

Scripts to analyze single actin filament dynamics TIRF experiments.

Filament analysis

The kymo2trace function converts a kymograph of a growing or shrinking filament into a change in length versus time trace.
- Click here for the Function
- Click here for the Tutorial

Drift detection and correction

The find_drift program takes a series of frames (i.e. a movie in tiff format) and uses an autocorrelation to determine the x- and y-drift over time.
- Click here for the Function

The fix_drift routine corrects for the drift found by find_drift program. It uses two inputs the raw movie and the drift file produced. It also requires a name for the corrected movie sequence. The size of the corrected movie will be n-1, as n is the number of frames in the original movie.
- Click here for the Function

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