Live cell imaging

We are regularly investigating intracellular processes in different contexts and with various key questions. On the Tracking Microscope, where we investigate particle dynamics, we simultaneously visualise the cell to reveal interactions of the particle with its surroundings and to understand the experiments in the cellular context. We use a multicolor confocal laser scanning microscope to examine protein-protein interactions in a cellular nucleus by scanning PIE-FCCS and are currently expanding our capabilities to simultaneously perform PIE-FCCS and FLIM measurements. On the Spinning Disc Microscope (SDM) we exploit the benefits in the z resolution of a confocal microscope to take fast z-stacks to accurately determine the structure of cellular mechanisms in 3 dimensions.

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