Virus LMU CENS BIN

Dr. Alvaro Crevenna

Research project

My main goal is to understand the assembly and functional dynamics of multi-subunit protein complexes; as isolated proteins and in reconstituted in vitro systems. Currently, I'm working towards understanding the biophysics of actin filament nucleation. I am using single molecule techniques (FCS, smFRET and TIRF) to study the dynamics and structure of actin/actin-nucleator complexes. I complement these studies with molecular dynamics and theoretical approaches.

Curriculum vitae

2010-present Independent postdoc, Priority Programme 1464 DFG, Principles and Evolution of actin nucleator complexes. Collaboration between MPI-B and LMU München. www.actindynamics.com
2006-2010 Postdoctoral fellow with Roland Wedlich-Söldner, Max Planck Institute of Biochemistry, Munich, Germany
2002-2006 PhD in Biophysics under Prof. Jonathon Howard. Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
1998-2002 Bachelor in Basic Biomedical Research, UNAM, Mexico

Grants

2013 BaCaTeC - Monitoring ubiquitin chain assembly with single molecule resolution. In collaboration with Raymond Deshaies, CalTec. www.bacatec.de/en/
2013 CeNS collaborative research project. In collaboration with Louis Reese, Theoretical Physics, LMU.
2013 CeNS travel grant
2013-2016 Priority Programme SPP 1464 DFG, Principles and Evolution of actin nucleator complexes, second period.
2010-2013 Priority Programme SPP 1464 DFG, Principles and Evolution of actin nucleator complexes, first period.

Publications

  • Crevenna A, Blank B, Maiser A, Enim D, Prescher J, Beck G, Kienzle C, Bartnik K, Habermann B, Pakdel M, Leonhardt H, Lamb DC, von Blume J
    Secretory cargo sorting by Ca2+-dependent Cab45 oligomerization at the trans-Golgi network.
    JCB. 2016; 213(3), 305-314 [doi link]
  • Jasnin M, Crevenna AH
    Interconnected, bundled and branched organization of Listeria actin tails supports stress-release mediated propulsion.
    2015
  • Geng Y, Kedrov A, Caumanns JJ, Crevenna AH, Lamb DC, Beckmann R, Driessen AJM
    Role of the cytosolic loop C2 and the C-terminus of YidC in ribosome binding and insertion activity.
    JBC. 2015 [doi link]
  • Crevenna AH, Arciniega M, Dupont A, Mizuno N, Kowalska K, Lange OF, Wedlich-Soeldner R, Lamb DC
    Side-Binding Proteins Modulate Actin Filament Dynamics.
    eLife. 2015 4 [doi link]
  • Kienzle C, Basnet N*, Crevenna AH*, Beck G, Habermann B, Mizuno N, von Blume J
    Cofilin recruits F-actin to SPCA-1 and promotes Ca2+-mediated secretory cargo sorting.
    J. Cell Biol. 2014 (5) 206 (* equal contribution)
  • Wang Q, Crevenna AH, Kunze I, Mizuno N
    Structural basis for the extended CAP-Gly domains of p150glued binding to microtubules and the implication for tubulin dynamics.
    Proc. Nat. Acad. Sci. 2014 (111), 11347-11352
  • Crevenna AH, Naredi-Rainer N, Schönichen A, Dzubiella J, Barber DL, Lamb DC, Wedlich-Söldner R
    Electrostatic control of actin filament nucleation and elongation kinetics.
    J Biol Chem. 2013 [doi link]
  • Koehler S, Schmoller K, Crevenna AH, Bausch A
    Regulating contractility of the actomyosin cytoskeleton by pH.
    Cell Reports 2012 Sep 27; 2(3):433-9
  • Schmoller KM, Kohler S, Crevenna AH*, Wedlich-Söldner R, Bausch AR*
    Modulation of cross-linked actin networks by pH.
    Soft Matter. 2012 (8), 9685 - 9690. (* corresponding author)
  • Crevenna AH, Naredi-Rainer N, Schulz JCF, Wedlich-Söldner R, Lamb DC, Dzubiella J
    Effects of Hofmeister Ions on the α-Helical Structure of Proteins.
    Biophys J 2012 (4) 102:907-915 [doi link]
  • Domìnguez-Escobar J, Chastanet A*, Crevenna AH*, Fromion V, Wedlich-Söldner R, Carballido-López R
    Processive movement of MreB-associated cell wall biosynthetic complexes in bacteria.
    Science 2011 Jul 8;333(6039):225-8. Epub 2011 Jun 2. (* equal contribution)
  • Yu JH, Crevenna AH, Bettenbuhl M, Freisinger T, Wedlich-Söldner R
    Cortical actin dynamics driven by formins and myosin V.
    J Cell Sci 2011 124(Pt9):1533-41
  • Crevenna AH, Cohen DN, Mathadil S, Fahmy K, Howard J
    Secondary Structure and Compliance of a Predicted Flexible Domain in Kinesin-1 Necessary for Co-operation of Motors.
    Biophys. J. 2008 Dec;95(11):5216-27
  • Riedl J*, Crevenna AH*, Kessenbrock K, Yu JH, Neukirchen D, Bista M, Bradke F, Jenne D, Holak TA, Werb Z, Sixt M, Wedlich-Söldner R
    Lifeact: a versatile marker to visualize F-actin.
    Nat. Methods 2008 5(7):605-7 (* equal contribution)
  • Schief WR, Clark RH, Crevenna AH, Howard J
    Inhibition of kinesin motility by ADP and phosphate supports a hand-over-hand mechanism.
    Proc. Nat. Acad. Sci. 2004 101 (5): 1183-8.
  • Basiuk VA, Crevenna AH, Montiel Molina HM
    Basis set effects on B3LYP Geometries and Energies: A Case Study of Interstellar Reaction HN-CH2 + 'C=N → H2N-C(')H-C=N.
    Int. J. Quantum Chem. 2002 Vol. 87, 101-109
  • Morales-Mulia S, Cardin V, Torres-Màrquez ME, Crevenna AH, Pasantes-Morales H
    Signaling pathway characterization of Taurine release induced by hyposmotic swelling.
    Neuochem. Inter. 2001 Feb; 38(2): 153-61

Patents

  • LifeAct - "Peptide for determining actin structures in living cells". European Patent No. 07023084.2 and 08007067.5, Commercialized by Ibidi GmbH.
  • Consultant for a company that commercializes actin products.

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